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Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag), Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag), Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag), Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag), Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag), Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag), Primary: Anti-SARS-CoV-2 N (orb1063082) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 52 kDa. |
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Sample: Lane 1~6: Recombinant SARS-CoV Nucleocapsid protein (1-422), Lane 7~12: Recombinant SARS-CoV-2 Nucleocapsid Protein (full length) (His tag), Primary: Anti-SARS-CoV-2 N (orb1063082) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 46/48 kD. |