MFN1 Recombinant Mouse Monoclonal Antibody, Clone: [G4A3], Unconjugated

Produktübersicht

• Artikelname: MFN1 Recombinant Mouse Monoclonal Antibody, Clone: [G4A3], Unconjugated
• Artikelnummer: BYT-ORB1172516
• Hersteller Artikelnummer: orb1172516
• Alternativnummer: BYT-ORB1172516-25,BYT-ORB1172516-50,BYT-ORB1172516-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ICC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: hfzo1, hfzo2, 2310002F04Rik, 6330416C07Rik, D3Ertd265e, HR2, mKIAA4032, Fzo1b, MFN1_HUMAN, MFN1, Fzo homolog, Transmembrane GTPase MFN1, 3.6.5.-, MFN1_MOUSE, MFN1_RAT, Mitochondrial transmembrane GTPase FZO1B,

Produktbeschreibung

MFN1 Recombinant Mouse Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1mg/ml
• Klon-Bezeichnung: [G4A3]
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: MFN1

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

Bilder

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HepG2, PC-3, PC-12, EL4.IL-12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 84 kDa, Observed MW: 84 kDa.

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MFN1) Monoclonal Antibody, Unconjugated (orb1172516) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.