GSSG is reduced to GSH using Glutathione Reductase (GR) for the determination of total glutathione. GSH reacts with 5,5-dithiobis-bis-(2-nitrobenzoic acid) to form 2-nitrobenzoic acid, which has an absorption peak at 412 nm.
Detektionsbereich:
0.04-4ug/mL
Sensitivitaet:
0.04ug/mL
Proben:
Animal and Plant Tissues, Bacteria, Cells and Serum
Anwendungsbeschreibung:
Application Notes: GSSG is reduced to GSH using Glutathione Reductase (GR). GSH reacts with 5,5-dithiobis-bis-(2-nitrobenoicacid) to form 2-nitrobenoicacid which has a characteristic absorption peak at 412 nm, the content of total glutathione is determined then. It can test samples of tissues, bacteria, fungus, cells, serums, ect
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