The kit provides a method for detecting Uric Acid (UA) concentration in animal tissues, serum, urine, and other biological fluids. Uricase catalyzes UA to produce H2O2, which then facilitates the formation of red quinone compounds via phenol and 4-aminoantipyrine.
Anwendungsbeschreibung:
Application Notes: The kit provides a simple method for detecting Uric Acid (UA) concentration in a variety of biological samples such as animal tissues, serum,urine and other biological fluids. In the assay, Uricase can catalyze UA to produce allantoin, CO2 and H2O2, H2O2 oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+, Fe3+ further condenses with phenol and 4-aminoantipyrine to form red quinone compounds which has a characteristic absorption peak at 505 nm. The UA content can be calculated by measuring the light absorption at this wavelength
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