GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG, glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+. NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not.
Anwendungsbeschreibung:
Application Notes: GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG, glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+, NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not, NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity
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