NCAM Recombinant Rabbit Monoclonal Antibody, Clone: [8C3], Unconjugated

Produktübersicht

• Artikelname: NCAM Recombinant Rabbit Monoclonal Antibody, Clone: [8C3], Unconjugated
• Artikelnummer: BYT-ORB1183968
• Hersteller Artikelnummer: orb1183968
• Alternativnummer: BYT-ORB1183968-100,BYT-ORB1183968-25,BYT-ORB1183968-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human, Mouse
• Immunogen: KLH conjugated synthetic peptide derived from human NCAM
• Konjugation: Unconjugated
• Alternative Synonym: CD56 antigen, Cell Adhesion Molecule Neural 1, MSK39, N CAM 120, N CAM 140, N CAM 180, NCAM 1, NCAM 120, NCAM 140, NCAM1, Neural cell adhesion molecule 1 120 kDa isoform, Neural cell adhesion molecule 1 140 kDa isoform, Neural cell adhesion molecule 1, NCAM1_HUMAN.

Produktbeschreibung

NCAM Recombinant Rabbit Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1mg/ml
• Klon-Bezeichnung: [8C3]
• Molekulargewicht: 92 kDa
• UniProt: P13591
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: NCAM1

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500

Bilder

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: SH-SY5Y, 2: Mouse cerebellum, 3: Rat cerebellum, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 95 kDa, Observed MW: 140-180 kDa.

Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NCAM) Monoclonal Antibody, Unconjugated (orb1183968) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NCAM) Monoclonal Antibody, Unconjugated (orb1183968) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human Prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NCAM) Monoclonal Antibody, Unconjugated (orb1183968) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NCAM) Monoclonal Antibody, Unconjugated (orb1183968) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NCAM) Monoclonal Antibody, Unconjugated (orb1183968) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NCAM, ) monoclonal Antibody, Unconjugated (orb1183968) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Western blot analysis of NCAM/CD56 on human brain tissue lysates with Rabbit anti-NCAM/CD56 antibody (orb1183968) at 1/2000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 95 kDa, Observed band size: 140/180 kDa, Exposure time: 30 seconds, 6% SDS-PAGE gel.

Western blot analysis of NCAM/CD56 on SH-SY5Y cell lysates with Rabbit anti-NCAM/CD56 antibody (orb1183968) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 95 kDa, Observed band size: 120/140 kDa, Exposure time: 2 minutes, 6% SDS-PAGE gel.