|
Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA with orb1239547, orb1239545, orb1239551, orb1239549, orb1239550, orb1239548, and orb1239546 antibodies at decreasing concentrations. |
|
Western blot analysis of LAG-3 in over expressing HEK293 cells using orb1239547, orb1239548, and orb1239546 antibodies at (A) 0.25 (B) 0.5 and (C) 1 µg/ml. |
|
Immunocytochemistry of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 µg/ml. |
|
Immunofluorescence of LAG-3 in over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 2 µg/ml. |
|
Immunofluorescence of LAG-3 in human spleen tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 10 µg/ml. |
|
Immunohistochemistry of LAG-3 in human lymphoma tissue using (A) orb1239547, (B) orb1239551, (C) orb1239549, (D) orb1239550, (E) orb1239548, (F) orb1239546, and (G) control mouse IgG antibody at 5 µg/ml. |
|
Flow cytometry analysis of LAG-3 over expressing HEK293 cells using (A) orb1239547, (B) orb1239545, (C) orb1239551, (D) orb1239549, (E) orb1239550, (F) orb1239548, (G) orb1239546, and (H) control mouse IgG antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells. |
|
A sandwich ELISA was performed using the anti-LAG3 mAbs orb1239547, orb1239545, orb1239551, orb1239549, orb1239550 and orb1239548 as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies. |