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Western blot analysis of PD-L2 in overexpressing 293 cells using orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 antibody at 0.5 and 1 µg/ml, respectively. Larger molecular weight bands represent more highly post-translationally modified PD-L2. |
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Immunocytochemistry of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 5 µg/ml. |
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Immunofluorescence of PD-L2 in overexpressing 293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 µg/ml. |
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Immunohistochemistry of PD-L2 in human colon carcinoma tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 µg/ml. |
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Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 10 µg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells. |
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Titration curve analysis of PD-L2 mAbs to detect recombinant PD-L2 in ELISA with orb1239853, orb1239856, orb1239859, orb1238786, and orb1239860 abs at decreasing concentrations. |
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Immunofluorescence of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 20 µg/ml. |
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Immunohistochemistry of PD-L2 in human tonsil tissue using (A) orb1239853, (B) orb1239856, (C) orb1239859, (D) orb1238786, (E) orb1239860, and (F) control mouse IgG antibody at 2 µg/ml. |