Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.8 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human B7-H4, Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human B7-H4, Fc Tag, low endotoxin at 0.5 ug/ml (100 uL/well) can bind Anti-B7-H4 MAb (Human IgG1) with a linear range of 0.2-8 ng/mL.
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