Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 27-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Canine CD47, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human SIRP alpha, Fc Tag (HPLC-verified) at 5 ug/ml (100 uL/well) can bind Canine CD47, His Tag with a linear range of 5-78 ng/mL.
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