Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 34.6 kDa. The protein migrates as 38-40 kDa and 41-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human C5a, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human C5a, Fc Tag at 2 ug/ml (100 uL/well) can bind Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL.
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