Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human Siglec-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Siglec-9, His Tag at 10 ug/ml (100 uL/well) on Nickel Coated plate, can bind Neu5Ac (a2-6) GalNAc-PAA-biotin with a linear range of 0.078-2.5 ug/ml.
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