Application Notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 62.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human Siglec-9, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Siglec-9, Fc Tag at 10 ug/ml (100 uL/well) on Diamond Protein A Protein, His Tag precoated (0.5 ug/well) plate, can bind Neu5Ac (a2-6) GalNAc-PAA-biotin with a linear range of 0.156-2.5 ug/ml.
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