Anti-PEX3 antibody (orb1238075) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminal of human PEX3. The immunogen is located within last 50 amino acids of PEX3.
PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
PEX3
WB Validation in Human Cell Lines. Loading: 15 µg of lysate. Antibodies: PEX3 orb1238075, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238075, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: PEX3 orb1238075, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of PEX3 in Human HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with orb1238075 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX3 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with orb1238075 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX3 in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with orb1238075 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunofluorescence Validation of PEX3 in Rat testis. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with orb1238075 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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