Anti-NCOA4 antibody (orb1238076) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
NCOA4
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of NCOA4 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with orb1238076 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of NCOA4 in Human lung. Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of NCOA4 in Mouse lung. Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of NCOA4 in Rat lung. Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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