Anti-SARS-CoV-2 (COVID-19) NSP10 antibody (orb1239080) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP10 protein. The immunogen is located in the last 50 amino acids of SARS-CoV-2 (COVID-19) NSP10.
SARS-CoV-2 (COVID-19) NSP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
Non-structural protein 10
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP10 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP10 antibody (orb1239080, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP10 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) NSP10 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP10 Antibody, orb1239080 A direct ELISA was performed using SARS-CoV-2 NSP10 recombinant protein (orb1231581) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) NSP10 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP10 recombinant protein (orb1231581). Antibodies: SARS-CoV-2 (COVID-19) NSP10, orb1239080, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL. Lane 2: 2 µg/mL. Lane 3: 4 µg/mL.
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