ACE2 Antibody, Unconjugated, Polyclonal

Artikelname:	ACE2 Antibody, Unconjugated, Polyclonal
Artikelnummer:	BYT-ORB1239144
Hersteller Artikelnummer:	orb1239144
Alternativnummer:	BYT-ORB1239144-0.02,BYT-ORB1239144-0.1
Hersteller:	Biorbyt
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	Anti-ACE2 antibody (orb1239144) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human ACE2. The immunogen is located within the last 50 amino acids of ACE2.
Konjugation:	Unconjugated
Alternative Synonym:	ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, SARS-CoV receptor, SARS-CoV-2 receptor

ACE2 Antibody

Klonalität:	Polyclonal
Konzentration:	1 mg/mL
Molekulargewicht:	Predicted: 93kDObserved: 130 kD (7 N-linked glycosylation)
NCBI:	068576
UniProt:	Q9BYF1
Puffer:	ACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:	Liquid
Target-Kategorie:	ACE2

	Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239146 (2 µg/mL), ACE2, orb1239144 (2 µg/mL), ACE2, orb1239144 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Immunofluorescence Validation of ACE2 in Human Kidney Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 with orb1239144 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
	Immunofluorescence Validation of ACE2 In Caco2 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Caco2 cells labeling ACE2 with orb1239144 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). Image showing membrane staining on Caco2 cells.
	Western Blot Validation in Human Tissues and Cell Line. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: ACE2, orb1239144 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
	Immunofluorescence Validation of ACE2 in Human Testis Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Immunofluorescence Validation of ACE2 in Human Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Immunofluorescence Validation of ACE2 in Mouse Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Immunofluorescence Validation of ACE2 in Rat Lung Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling ACE-2 with orb1239144 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
	Immunohistochemistry Validation of ACE2 in Human Kidney Tissue. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody (orb1239144) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.