Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.The immunogen is located within amino acids 340 - 390 of Albumin.
Konjugation:
Unconjugated
Alternative Synonym:
Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin
Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
ALB
Anwendungsbeschreibung:
Application Notes: WB: 1-2 µg/mL, IHC-P/IF: 10-20 µg/mL, IHC-P: 2-10 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence in mouse samples, Immunohistochemistry in human, mouse, and rat samples, . All other applications and species not yet tested
Western Blot Validation in Human Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Liver. Loading: 10 µg of lysates per lane. Antibodies: Albumin, orb1239194, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of Albumin in Human Liver. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Albumin in Mouse Liver. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (orb1239194) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of Albumin in Rat Liver. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (orb1239194) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of Albumin in Mouse Liver. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with orb1239194 at 20 /mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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