Anti-NPTX2 antibody (orb1239671) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2. The immunogen is located within amino acids 170 - 220 of NPTX2.
NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
NPTX2
Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (2 µg/mL), NPTX2 orb1271003 (4 µg/mL), and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: NPTX2 orb1239671 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. A: Absence of blocking peptide B: Presence of blocking peptide.
Immunofluorescence Validation of NPTX2 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling NPTX2 with orb1239671 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry Validation of NPTX2 in Human Brain. Immunohistochemical analysis of paraffin-embedded human brain using anti-NPTX2 antibody (orb1239671) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of NPTX2 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling NPTX2 with orb1239671 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunohistochemistry Validation of NPTX2 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-NPTX2 antibody (orb1239671) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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