PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Formulierung:
Liquid
Target-Kategorie:
CD274
Anwendungsbeschreibung:
Application Notes: PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 µg/mL. For immunofluorescence start at 5µg/mL.Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested
Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 µg/ml
Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 µg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 µg/mL.
Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 µg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining
Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 µg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining
Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 µg/mL. Red: PDL1 Antibody [1D7] (orb1239818) Blue: DAPI staining
Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 µg/mL.
Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 µg/mL.
A sandwich ELISA was performed using the anti-PD-L1 mAb orb1239818 (5 µg/ml) as the capture antibody. Biotin-labeled anti-PD-L1 mAb orb1238288 (1 µg/ml) and streptavidin-HRP (0.1 µg/ml) were used for detection. Detection range is from 10 ng to 20 pg.
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