TWEAK Antibody is supplied in PBS containing 0.02% sodium azide.
Formulierung:
Liquid
Target-Kategorie:
TNFSF12
Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: TWEAK orb1240271 (A: 1 µg/mL and B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK orb1240271 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL, Lane 3: 2 µg/mL and Lane 4: 4 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kD.
Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: TWEAK orb1240271 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: TWEAK orb1240271 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of TWEAK in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK antibody (orb1240271) at 10 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of TWEAK in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK antibody (orb1240271) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of TWEAK in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK with orb1240271 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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