Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
Ptgds
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26kDa band observed in Mouse Brain and Mouse fetal Brain lysates (calculated MW of 21.1kDa according to NP_032989.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/mL
orb1248723 (0.1 ug/ml) staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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