Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
HMHA1
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 200kDa band in lysates of cell lines Jurkat and Molt4 after 0.05µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kDa according to NP_036424.2. The 200kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5µg/mL
orb1248732 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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