Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
Clu
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in Mouse Testis lysates (calculated MW of 51.7kDa according to NP_038520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, PMID: 11560857). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch
orb1248755 (0.3 ug/ml) staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
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