Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
KLF13
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30+28kDa bands observed in Human Heart lysates (calculated MW of 31.2kDa according to NP_057079.2). Recommended concentration: 0.1-0.3µg/mL. The 28kDa is explained by partly proteolysis. Both bands were blocked by the immunnizing peptide
orb1248758 (0.1 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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