Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
SOX11
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 65kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 46.7kDa according to NP_003099.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Dictor et al, Haematologica. 2009 Nov,94(11):1563-8. PMID: 19880779). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3, Mouse and Rat Brain for this particular batch
orb1248783 (0.5 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
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