Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
ATP2B1
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 135kDa according to NP_001673.2). Recommended concentration: 0.03-0.1µg/mL
orb1248809 (0.03 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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