Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
TFB2M
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in Human Lung lysates (calculated MW of 45.3kDa according to NP_071761.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 5ug/ml
orb1249265 (0.1 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1249265 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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