Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Formulierung:
Liquid
Target-Kategorie:
SPG20
Anwendungsbeschreibung:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 72.8kDa according to NP_055902.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA
orb1249374 (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249374 (2 ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.
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