Western blot analysis of extracts of various cell lines, using PNKD antibody (orb1254265) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using PNKD antibody (orb1254265) at dilution of 1:100. Blue: DAPI for nuclear staining.
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