Western blot analysis of extracts of 22Rv1 cells, using TMPRSS2 antibody (orb1258355) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach cancer using TMPRSS2 antibody (orb1258355) at dilution of 1:200 (40x lens).
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