Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.
Application Notes: ELISA:Sandwich:To detect Human IL-17D by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyts Polyclonal Anti-Human IL-17D (38-266) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17D.Western Blot:To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-17D is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect Rat GM-CSF by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti- Rat GM-CSF (orb1272634) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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