Produced from sera of rabbits immunized with highly pure recombinant Murine TRAIL. Murine TRAIL specific antibody was purified by affinity chromatography and then biotinylated.
Konjugation:
Biotin
Alternative Synonym:
TL2, Ly81, Trail, APO-2L, AI448571, A330042I21Rik, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL
Application Notes: ELISA:Sandwich:To detect Murine TRAIL by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyts Polyclonal Anti-Murine TRAIL (38-259) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TRAIL.Western Blot:To detect Murine TRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for recombinant Murine TRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions
To detect Murine TRAIL by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine TRAIL (orb1272648) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TRAIL.
To detect Murine TRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Murine TRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine TRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Murine TRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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