Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
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