YBX1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1274422
Artikelname: YBX1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1274422
Hersteller Artikelnummer: orb1274422
Alternativnummer: BYT-ORB1274422-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
Konjugation: Unconjugated
Alternative Synonym: NSEP1, YB1, BP-8, CSDB, DBPB, YB-1, CSDA2, NSEP1, NSEP-1, MDR-NF1
YBX1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 49kDa
UniProt: P67809
Puffer: Liquid
Formulierung: Liquid
Target-Kategorie: YBX1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (orb1274422) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (orb1274422) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using YBX1 antibody (orb1274422) at dilution of 1:100. Blue: DAPI for nuclear staining.