AIFM1 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: BYT-ORB1274462
Artikelname: AIFM1 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: BYT-ORB1274462
Hersteller Artikelnummer: orb1274462
Alternativnummer: BYT-ORB1274462-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC, IP, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
Konjugation: Unconjugated
Alternative Synonym: AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1
AIFM1 Antibody
Klonalität: Polyclonal
Konzentration: batch dependent
Molekulargewicht: Observed: 67kDa
UniProt: O95831
Puffer: Liquid
Formulierung: Liquid
Target-Kategorie: AIFM1
Anwendungsbeschreibung: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using AIF antibody (orb1274462). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using AIF antibody (orb1274462) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using AIF antibody (orb1274462) at dilution of 1:200. Blue: DAPI for nuclear staining.