Western blot analysis of extracts of various cell lines, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using AIF antibody (orb1274462). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using AIF antibody (orb1274462) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using AIF antibody (orb1274462) at dilution of 1:200. Blue: DAPI for nuclear staining.
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