This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Formulierung:
Liquid
Anwendungsbeschreibung:
Application Notes: ELISA (Quality Tested by ProSci)
Coating: Purified Recombinant SARS-CoV-2 Spike NTD (Clone 2146) was coated at concentration of 1 ul/well, 100 ul/well overnight 2-8 °C. Sample: Purified rSARS CoV-2 Trimeric Spike Protein at 0.5 µg/mL and serially diluted (2-fold) to 0.98 ng/ml, 100ul/well for 1 hour at 37 °C. Detection: Anti-SARS-CoV-2 Spike RBD (Clone 2196) conjugated to HRP diluted to 0.1 ug/ml, 100 ul/well for 1 hour at 37 °C. Substrate: TMB, 100 ul/well for 15 min, at room temperature followed by 450 nm stop solution, 50ul/well.
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