Aurora A/AURKA Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Aurora A/AURKA Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB1290027
• Hersteller Artikelnummer: orb1290027
• Alternativnummer: BYT-ORB1290027-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: E.coli-derived human Aurora A/AURKA recombinant protein (Position: K23-S403).
• Konjugation: Unconjugated
• Alternative Synonym: Probable ATP-dependent RNA helicase DDX58, 3.6.4.13, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein, RIG-I, DDX58

Produktbeschreibung

Anti-Aurora A/AURKA Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molekulargewicht: 46 kDa
• UniProt: O14965
• Formulierung: Lyophilized
• Target-Kategorie: Hemoglobin subunit alpha

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human ELISA, 0.1-0.5 µg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml

Bilder

Flow Cytometry analysis of HepG2 cells using anti-Aurora A/AURKA antibody. Overlay histogram showing HepG2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aurora A/AURKA Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Aurora A/AURKA Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Aurora A/AURKA Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Aurora A/AURKA Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Aurora A/AURKA Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected in a paraffin-embedded section of human renal oncocytoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Aurora A/AURKA Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Aurora A/AURKA using anti-Au

IHC analysis of Aurora A/AURKA using anti-Aurora A/AURKA antibody. Aurora A/AURKA was detected