Human 14-3-3 Protein Gamma (YWHAG) ELISA Kit

Artikelnummer: BYT-ORB1291376
Artikelname: Human 14-3-3 Protein Gamma (YWHAG) ELISA Kit
Artikelnummer: BYT-ORB1291376
Hersteller Artikelnummer: orb1291376
Alternativnummer: BYT-ORB1291376-48, BYT-ORB1291376-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: 14 3 3 gamma , 14 3 3 protein gamma , 14 3 3 protein gamma subtype , 14 3 3gamma , 14-3-3 protein gamma , 1433G_HUMAN , 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide , KCIP 1 , KCIP-1 , KCIP1 , N-terminally processed , Protein kinase C inhibitor protein 1 , Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide , Ywhag
Human YWHAG (14-3-3 protein gamma) ELISA Kit
Konzentration: 20 ng/mL
Detektionsbereich: 0.32-20 ng/mL
Sensitivitaet: 0.126 ng/mL
UniProt: P61981
Proben: Serum, plasma, tissue homogenates and other biological fluids.
Anwendungsbeschreibung: Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human YWHAG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human YWHAG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human YWHAG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human YWHAG in the samples is then determined by comparing the OD of the samples to the standard curve