Human Free ProstateSpecific Antigen (F-PSA) ELISA Kit

Artikelnummer: BYT-ORB1291378
Artikelname: Human Free ProstateSpecific Antigen (F-PSA) ELISA Kit
Artikelnummer: BYT-ORB1291378
Hersteller Artikelnummer: orb1291378
Alternativnummer: BYT-ORB1291378-48, BYT-ORB1291378-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Applikation: ELISA
Spezies Reaktivität: Human
Alternative Synonym: F-PSA
Human F-PSA (Free ProstateSpecific Antigen) ELISA Kit
Konzentration: 2000 pg/mL
Detektionsbereich: 31.25-2000 pg/mL
Sensitivitaet: 17.31 pg/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human F-PSA in the samples is then determined by comparing the OD of the samples to the standard curve