THRB Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: THRB Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1291533
• Hersteller Artikelnummer: orb1291533
• Alternativnummer: BYT-ORB1291533-100,BYT-ORB1291533-200,BYT-ORB1291533-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human
• Immunogen: Recombinant human THRB protein (209-461/461aa)
• Konjugation: Unconjugated
• Alternative Synonym: Thyroid Hormone Receptor beta, Avian erythroblastic leukemia viral(v erb a) oncogene homolog 2, C ERBA 2, C ERBA BETA, c-erbA-2, c-erbA-beta, ERBA 2, ERBA BETA, ERBA2, Erythroblastic leukemia viral(v erb a) oncogene homolog 2 avian, generalized resistance to thyroid hormone, GRTH, MGC126109, MGC126110, NR1A2, Nuclear receptor subfamily 1 group A member 2, Oncogene ERBA2, PRTH, THB_HUMAN, THR 1, THR1, THRB 1, THRB 2, THRB2, Thyroid hormone nuclear receptor beta variant 1, Thyroid hormone receptor beta 1, Thyroid hormone receptor beta 2, Thyroid hormone receptor beta, Thyroid hormone receptor, beta(erythroblastic leukemia viral(v erb a) oncogene homolog 2, avian), THbeta1, THbeta2.

Produktbeschreibung

THRB Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 53 kDa
• UniProt: P10828
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: THRB

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:200-800, IHC-F=1:400-800, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000

Bilder

Blank control (black line): A431. Primary Antibody (green line): Rabbit Anti-THRB antibody (orb1291533), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): A431. Primary Antibody (green line): Rabbit Anti-THRB antibody (orb1291533), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-THRB antibody (orb1291533), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-THRB antibody (orb1291533), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human THRB Antibody, the EC50 is 133.9 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human THRB protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human THRB Antibody, the EC50 is 942.9 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (orb1291533) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human thyroid gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (THRB) Polyclonal Antibody, Unconjugated (orb1291533) at 1:200 overnight at 4C

IHC-P analysis of human thyroid gland using THRB Polyclonal Antibody at 1:200 dilution.