Synthetic peptide / encompassing a sequence within the C-terminus region.
Konjugation:
Unconjugated
Rabbit polyclonal antibody to Fibronectin (C-term)
Klonalität:
Polyclonal
Target-Kategorie:
Fibronectin 1 / FN1 (C-term)
Application Verdünnung:
Western Blot 1:500 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200
All lanes: Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294256 overnight at 4C.
Anti-Fibronectin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was incubated with orb1294256 overnight at 4C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294256 anti-Fibronectin antibody (red) at 1:200 and 4C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.
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