NI-57 is a potent small molecule inhibitor targeting the BRPF family of proteins, exhibiting IC50 values of 3.1 nM for BRPF1, 46 nM for BRPF2/BRD1, and 140 nM for BRPF3. This compound is a valuable chemical probe for epigenetic research, enabling the investigation of BRPF function in chromatin regulation and gene expression in both cellular and biochemical assays.