WNT5A Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: WNT5A Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB13762
• Hersteller Artikelnummer: orb13762
• Alternativnummer: BYT-ORB13762-100,BYT-ORB13762-200,BYT-ORB13762-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human WNT5A (301-381/381aa)
• Konjugation: Unconjugated
• Alternative Synonym: wingless-related MMTV integration site 5A, hWNT 5A, hWNT5A, Protein Wnt 5a, Protein Wnt5a, Wingless type MMTV integration site family member 5A, Wnt-5a, WNT 5A protein precursor, WNT5A protein precursor, WNT5A_HUMAN.

Produktbeschreibung

WNT5A Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 35 kDa
• UniProt: P41221
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: WNT5A

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Bilder

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-WNT5A antibody (orb13762), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (WNT5A) Polyclonal Antibody, Unconjugated (orb13762) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Embryo (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Uterus (Mouse) Lysate at 40 ug, Lane 4: Uterus (Rat) Lysate at 40 ug, Lane 5: Ovary (Rat) Lysate at 40 ug, Lane 6: Vas deferens (Rat) Lysate at 40 ug, Primary: Anti-WNT5A (orb13762) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.

Sample: Ovary (Mouse) Lysate at 30 ug, Primary: Anti-WNT5A (orb13762) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 47 kD.

Sample: Uters (Mouse) Lysate at 30 ug, Primary: Anti-WNT5A (orb13762) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 47 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-WNT5A Polyclonal Antibody, Unconjugated (orb13762) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemical analysis of paraffin-embedded brain tissue of mice using WNT5A antibody

IHC-P of brain tissue of mice (WNT5A antibody at 1:1000)