This kit provides seven specially formulated ELISA solutions and a template for the initial development and optimization of antigen-down immunoassays by both novice and experienced users. The kit contains enough reagent for ten 96-well ELISA plates.
Anwendungsbeschreibung:
Application Notes: 1. Prepare samples., 2. Create controls with CCCP., 3. Dilute 10X Assay Buffer 1:10 with diH2O., 4. Reconstitute MitoPT TMRE with DMSO., 5. Dilute MitoPT TMRE with 1X Assay Buffer., 6. Add MitoPT TMRE to each sample., 7. Incubate 15-30 minutes., 8. Wash cells: add 1X Assay Buffer and spin cells., 9. Remove supernatant and resuspend cells for analysis., 10. Anazlyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT TMRE excites at 549 nm and emits at 574 nm (orange)
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