Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The DAP group phosphonylates the active site serine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader.
Proben:
Cell culture, tissue
Anwendungsbeschreibung:
Application Notes: 1. Add 1 part Antigen-Down HRP Conjugate Stabilizer to 4 parts deionized water and mix for 5-15 minutes., 2. Add the anti-IgG-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes., 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8C for use at a later date. 1X working conjugate dilutions can be stored at 2-8C and protected from light, providing a useful shelf-life of 6-12 months
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