P2Y9 Polyclonal Antibody, Unconjugated, Rabbit

Produktübersicht

• Artikelname: P2Y9 Polyclonal Antibody, Unconjugated, Rabbit
• Artikelnummer: BYT-ORB1420914
• Hersteller Artikelnummer: orb1420914
• Alternativnummer: BYT-ORB1420914-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Porcine, Rat
• Konjugation: Unconjugated
• Alternative Synonym: Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, G-protein coupled receptor 23, P2Y purinoceptor 9, P2Y9, Purinergic receptor 9, Lpar4, Gpr23, Lpa4, P2y9

Produktbeschreibung

Rabbit polyclonal antibody to P2Y9.

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: Q8BLG2

Bilder

Sample: Brain (mouse) Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: HL-60 Cell (Human) Lysate at 30 ug. Primary: Anti-P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 55 kD. Observed band size: 55 kD.

Sample: k562 (human) cell Lysate at 40 ug. Primary: Anti- P2Y9 at 1/300 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 48 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug. Ovary (Mouse) Lysate at 40 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Sample: HL-60 (Human) Cell Lysate at 30 ug. K562 (Human) Cell Lysate at 30 ug. Primary: Anti-P2Y9 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 42 kD. Observed band size: 57 kD.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.