Goat IgG F(c) fragment Rhodamine conjugated, Goat IgG F(c) fragment TRITC conjugated
Goat IgG F(c) Rhodamine Antibody
Konzentration:
1.0 mg/mL
Puffer:
0.01% (w/v) Sodium Azide
Quelle:
Goat
Reinheit:
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat IgG, anti-Goat IgG F(c) and anti-Goat Serum. No reaction was observed against anti-Goat IgG F(ab)2 or anti-Papain.
Formulierung:
Lyophilized
Anwendungsbeschreibung:
Biological Origin: Goat. Application Notes: Goat IgG F(c) fragment Fluorescein conjugated has been tested in dot blot and SDS-Page and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, and utilizing various commercial platforms
Dot Blot of Rhodamine Conjugated Goat IgG Fc. Dotted Rhodamine Conjugated Goat IgG Fc with following concentrations. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng Primary antibody: none Secondary antibody: none Block: orb348637 for 1 HR at RT.
SDS-PAGE of Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane M: 5 µl Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule Rhodamine Conjugated (p/n orb346100). Lane 2: Reduced Goat IgG F(c) Fragment (p/n orb2652765). Lane 3: Reduced Goat IgG F(ab) Fragment (p/n orb2652763). Lane 4: Reduced Goat IgM Whole Molecule (p/n orb346110). Load: 1 µg for IgG, F(c) and F(ab), 3 µg for IgM. Predicted/Observed size: IgG at 50 and 25 kDa, F(c) at 25 kDa, F(ab) at 25 kDa, IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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