Lyophilized from a 0.2 µm filtered solution of 20mM Tris-HCl,500mM NaCl,5%Trehalose,5%Mannitol,0.01%tween80,1mM EDTA,pH8.0.
Reinheit:
Greater than 95% as determined by reducing SDS-PAGE.
Formulierung:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target-Kategorie:
pro-Beta
Canine PD-1 Protein, mFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/mL (100 µL/well) Canine PD-1 Protein, mFc Tag (orb2321234) can bind Anti-Canine PD-1 (INTERVET 4F12) mAb (orb2321976) in a linear range of 0.64-80 ng/ml.
ELISA plate pre-coated by 2 µg/mL (100 µL/well) Canine PD-1 Protein, mFc Tag (orb2321234) can bind Canine PD-L1 Protein, hFc Tag (orb2321231) in a linear range of 16-2000 ng/ml.
Flow cytometry analysis with 1 µg/mL Canine PD-1 Protein, mFc Tag (orb2321234) on Expi293 cells transfected with Canine PD-L1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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