HSP27 Antibody (Biotin), Clone: [5D12-A12], Mouse, Monoclonal

Produktübersicht

• Artikelname: HSP27 Antibody (Biotin), Clone: [5D12-A12], Mouse, Monoclonal
• Artikelnummer: BYT-ORB147517
• Hersteller Artikelnummer: orb147517
• Alternativnummer: BYT-ORB147517-200
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, FC, ICC, IF, IHC, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Full length human HSP27
• Konjugation: Biotin
• Alternative Synonym: HSPB1, HSP27, HSP25, HSP28, Heat shock protein beta-1, Heat shock 27 kDa protein, MKBP, DMPK-binding protein, SRP27, 28kDa heat shock protein, CMT2F

Produktbeschreibung

Mouse monoclonal to Hsp27 (Biotin). Hsp27s belong to an abundant and ubiquitous family of Hsp27s belong to an abundant and ubiquitous family of...

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [5D12-A12]
• Molekulargewicht: 27kDa
• NCBI: 001531
• UniProt: P04792
• Puffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target-Kategorie: HSP27

Anwendungsbeschreibung

• Application Verdünnung: WB (1:2000), ICC/IF (1:100)
• Anwendungsbeschreibung: Application Notes: 0.5 µg/ml of SMC-161 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 5D12-A3. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 5D12-A3. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull heterogeneous staining, some perinuclear, some nuclear and some cytoplasmic staining.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 5D12-A3. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Western Blot analysis of Human Cell lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 5D12-A3. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.