Mouse Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit

Produktübersicht

• Artikelname: Mouse Extracellular Signal Regulated Kinase 1 (ERK1) ELISA Kit
• Artikelnummer: BYT-ORB1496499
• Hersteller Artikelnummer: orb1496499
• Alternativnummer: BYT-ORB1496499-48,BYT-ORB1496499-96
• Hersteller: Biorbyt
• Kategorie: Kits/Assays
• Spezies Reaktivität: Mouse
• Alternative Synonym: HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, MAPK3, ERT2, Mitogen-Activated Protein Kinase 3, Insulin-stimulated MAP2 kinase, Microtubule-associated protein 2 kinase

Produktbeschreibung

Mouse ERK1 ELISA Kit

Produkteigenschaften

• Konzentration: 5000 pg/mL
• Detektionsbereich: 6.86-5000 pg/mL
• Sensitivitaet: 3.05 pg/mL
• UniProt: Q63844
• Proben: Serum, plasma, tissue homogenates and other biological fluids

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ERK1 in the samples is then determined by comparing the OD of the samples to the standard curve

Bilder

orb1496499